Journal:

Article Title: Aponecrotic, antiangiogenic and antiproliferative effects of a novel dextran derivative on breast cancer growth in vitro and in vivo

doi: 10.1038/sj.bjp.0704645

Figure Lengend Snippet: Inhibition of MCF-7ras tumour angiogenesis by NaPaC, CMDB and NaPa. Microvessel staining of tumours untreated (A) and treated with 150 mg kg−1 CMDB (B), 40 mg kg−1 NaPa (C) or 15 mg kg−1 NaPaC (D), twice a week, for 7 weeks, was performed using GSL-1 lectin. Necrotic areas in panel d are indicated with the asterisks. Magnification ×250 was used for panels A, B and C, and magnification ×100 was applied for panel D. The representative microvessels in panels A – C are marked with the arrows.

Article Snippet: Endothelial cells were specifically labelled with GSL-1 isolectin B4 (Vector Laboratories, Burlingame, CA, U.S.A.).

Techniques: Inhibition, Staining

Journal: The Journal of nutritional biochemistry

Article Title: A high docosahexaenoic acid diet alters lung inflammation and recovery following repetitive exposure to aqueous organic dust extracts

doi: 10.1016/j.jnutbio.2021.108797

Figure Lengend Snippet: Changes in the Arg-1 positive macrophages after repetitive HDE exposure and DHA diet supplementation in the lung at three weeks (A-D) and after the recovery period (E-H). Formalin fixed and paraffin embedded lung sections were stained for Arg-1 and GSL, M2 and M1/M2 macrophage marker in tissues from mice receiving control or DHA diet for four weeks prior to three-week HDE exposure. Images were obtained by epifluorescence microscope Echo Revolve with 20X objective in FITC (Arg-1) and Cy5 (GSL) channels. Between 5-7 images for airways and lung parenchyma were obtained. The fluorescence intensity of the whole image was determined by quantifying integrated density/area in Image J. Quantification of Arg-1 immunofluorescence signal around the airways and parenchyma at three-weeks (C, D) and at the recovery period (G, H) are shown as number of Arg-1/ GSL positive cells and fluorescence intensity. Data are mean ± standard error of the mean. * P <.05, ** P <.01, *** P <.001.

Article Snippet: The M2 macrophage markers Ym1 and Arginase-1 (Arg-1) and a lectin recognizing both M1/M2 macrophages, GSL (Griffonia Simplicifolia Lectin-1 isolectin B4, biotinylated, Vector Laboratories, # B-1205-.5), were detected using immunofluorescence staining and microscopy.

Techniques: Staining, Marker, Microscopy, Fluorescence, Immunofluorescence